Composite

Part:BBa_K1217008:Design

Designed by: Ma Tsz Shan   Group: iGEM13_Hong_Kong_HKU   (2013-09-15)


Localizing polyphosphate kinase (ppk1) into native Eut microcompartment (BBa_K311004)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2631
    Illegal NotI site found at 3541
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2289
    Illegal BglII site found at 2844
    Illegal BglII site found at 3754
    Illegal XhoI site found at 2639
    Illegal XhoI site found at 3549
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3468
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3056
    Illegal SapI.rc site found at 366


Design Notes

Source

Kingella oralis (ATCC 51147)

References